Hepatoprotective Activity of Adhatoda vasica and Vitex negundo Leaf Extracts Against Carbon Tetrachloride Induced Hepatotoxicity in Rats

Liver diseases such as jaundice, cirrhosis etc. are very common worldwide and traditional medicine are proving to be useless or less effective against them. Therefore workers are trying to find out alternate medicine or formulations to combat these diseases. In the present study hepatoprotective activity of aqueous extract of Adhatoda vasica and Vitex negundo on total protein, bilirubin, aspartate aminotransferase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) in CCl intoxicated rats was studied. Administration 4 of CCl showed significant increase (p < 0.01) in elevation of liver marker enzymes in serum namely 4 AST (107.2 ± 13.17), ALT (206.2 ± 28.82), ALP (508.22 ± 10.22), total bilirubin (2.61 ± 0.27) and significantly decreased total protein (5.2 ± 0.085) when compared to normal. Av and Vn at 250 mg/kg and 500 mg/kg body weight showed significant increase in total protein (8.0 ± 0.05, 7.11 ± 0.044; 7.02 ± 0.02, 6.23 ± 0.03) when compared to CCl treated rats. Both Av and leaf extracts lowered enzymes levels, which is a designation of 4 hepatoprotective action of the extracts. The serum AST, ALT and ALP levels are reliable markers of liver function. Thus the present study concludes the aqueous leaf extract of Adhatoda vasica and Vitex negundo to possess hepatoprotective activity.